BiohacksAI/Mechanism Hypothesis/Methotrexate

Methotrexate — Mechanism Gap Analysis

HYPOTHESIS_ENGINE_v1.0 · Corpus v20260227-01 · Deterministic graph-traversal

Known Targets

CYP2C19CYP2D6CYP2C9CYTOCHROMETDP1THRBESRRANFE2L2SYKTYROSINE-PROTEINCHAINPGRRATTUSNSD2MUSHISTONE-LYSINENUCLEARTP53ABCB1EUCHROMATICHOMOIFNGPPARGVDRNTMT1ATXN2NR1H4MDM2MDM4IDH1BRCA1POLBBARD1POLINR5A1MPHOSPH8POLHNR5A2NCOA3TNFRSF10BABCC3NCOA1TYMSDHFRJAK3TYK2TLR4FOLATEFOLR1KLK7SLC19A1SLC46A1SLCO1A8ATICFPGSGARTHMGB1SLC17A5

58 known targets mapped from PubChem bioassay data

Pathway Context

SUMOylation10 targets overlapping

SUMO E3 ligases SUMOylate target proteins10 targets overlapping

Cell Cycle8 targets overlapping

Uncovered Pathway Targets

216 total

ARAURKAAURKBBIRC5BLMCREBBPCTBP1DNMT1DNMT3BEIF2AK2+206 more…

Genes present in the top pathway union that Methotrexate does not directly modulate.

Top Stack Candidates

Bortezomib

#1 gap-filler · 45 targets covered · evidence score 44.2

score 12,633.619

ARESR1HDAC1HDAC2MITFNFKB2NR1I2PARP1RARARELAADRM1HDAC8+33

Quercetin

#2 gap-filler · 31 targets covered · evidence score 43.4

score 6,646.183

ARAURKBBLMESR1NFKB2NR1I2NR3C1RELARXRATOP1TOP2AABL1+19

657 more candidates · full stack optimisation locked

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BiohacksAI maps biological mechanisms. It does not recommend compounds. Results generated by HYPOTHESIS_ENGINE_v1.0.